2017年6月7日， 國際著名學術期刊《美國科學院院刊》線上發表了浙江大學昆蟲科學研究所王曉偉教授課題組題為“Vector development and vitellogenin determine the transovarial transmission of begomoviruses”的研究論文， 研究報導了歷時5年潛心研究發現的植物病毒傳播的一個重要通道。 昆蟲所博士研究生衛靜和何亞洲為文章的共同第一作者， 王曉偉教授為通訊作者。
在目前已知的近千種植物病毒中， 約三分之二的病毒主要依賴媒介昆蟲進行傳播， 因此研究植物病毒通過媒介昆蟲傳播的機制具有重要意義。 雙生病毒占目前已記錄的全部植物病毒三分之一， 主要通過媒介昆蟲煙粉虱傳播。
原文連結：
原文摘要：
The majority of plant viruses are transmitted by insect vectors between hosts, and transovarial transmission of viruses from vector parents to offspring has great significance to their epidemiology. Begomoviruses are transmitted by the whitefly Bemisia tabaci in a circulative manner and are maintained through a plant–insect–plant cycle. Other routes of begomovirus transmission are not clearly known. Here, we report that transovarial transmission from female whiteflies to offspring often happens for one begomovirus, Tomato yellow leaf curl virus (TYLCV), and may have contributed significantly to its global spread. We found that TYLCV entry of the reproductive organ of its vector mainly depended on the developmental stage of the whitefly ovary, and the transovarial transmission of TYLCV to offspring increased with whitefly adult age. The specific interaction between virus coat protein (CP) and whitefly vitellogenin (Vg) was vital for virus entry into whitefly ovary. When knocking down the expressionof Vg, the entry of TYLCV into ovary was inhibited and the transovarial transmission efficiency decreased. In contrast, another begomovirus, Papaya leaf curl China virus (PaLCuCNV), CP did not interact with whitefly Vg, and PaLCuCNV could not be transovarially transmitted by whiteflies. We further showed that TYLCV could be maintained for at least two generations in the absence of virus-infected plants, and the adult progenies were able to infect healthy plants in both the laboratory and field. This study reports the transovarial transmission mechanism of begomoviruses, and it may help to explain the evolution and global spread of some begomoviruses.
作者：王曉偉 點擊：次